Preparation of phosphatidyl inositol from baker's yeast.
نویسنده
چکیده
Phosphatidyl inositol and a cardiolipin-like phospholipid survive the autolysis which is induced in yeast by treating it with toluene, whereas other phospholipids are extensively degraded. Lipid from autolyzed yeast was suspended in isopropanol to extract lecithin and neutral lipid. The insoluble phosphatidyl inositol and cardiolipin were then separated by chromatography on silicic acid.
منابع مشابه
Removal of the sphingolipid impurity from preparations of yeast phosphatidyl inositol.
A complex sphingolipid containing inositol and mannose, present in lipid extracted from toluene-autolyzed baker's yeast, was eluted from silicic acid columns immediately after phosphatidyl inositol, and was the main nitrogenous impurity in crude preparations of this phospholipid. Nitrogenfree phosphatidyl inositol was obtained by rechromatography on alumina. Modifications to the chromatographic...
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ورودعنوان ژورنال:
- Journal of lipid research
دوره 7 3 شماره
صفحات -
تاریخ انتشار 1966